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Title: | Synchronized Electromechanical Shock Wave-Induced Bacterial Transformation |
Authors: | Bhatt, Geeta |
Keywords: | Mechanical Engineering Electromechanical |
Issue Date: | May-2019 |
Publisher: | ACS |
Abstract: | We report a simple device that generates synchronized mechanical and electrical pressure waves for carrying out bacterial transformation. The mechanical pressure waves are produced by igniting a confined nanoenergetic composite material that provides ultrahigh pressure. Further, this device has an arrangement through which a synchronized electric field (of a time-varying nature) is initiated at a delay of ≈85 μs at the full width half-maxima point of the pressure pulse. The pressure waves so generated are incident to a thin aluminum–polydimethylsiloxane membrane that partitions the ignition chamber from the column of the mixture containing bacterial cells (Escherichia coli BL21) and 4 kb transforming DNA. A combination of mechanical and electrical pressure pulse created through the above arrangement ensures that the transforming DNA transports across the cell membrane into the cell, leading to a transformation event. This unique device has been successfully operated for efficient gene (∼4 kb) transfer into cells. The transformation efficacy of this device is found comparable to the other standard methods and protocols for carrying out the transformation. |
URI: | https://pubs.acs.org/doi/full/10.1021/acsomega.9b00202 http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/12215 |
Appears in Collections: | Department of Mechanical engineering |
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