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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rao, V. Ramgopal | - |
dc.date.accessioned | 2023-10-20T07:14:59Z | - |
dc.date.available | 2023-10-20T07:14:59Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acsomega.1c01702 | - |
dc.identifier.uri | http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/12549 | - |
dc.description.abstract | The development of a nanoparticle-based opticalplatform has been presented as a biosensor for detecting target-specific plant virus DNA. The binding dynamics of goldnanoparticles has been studied on the amine-functionalized surfaceby the attenuated total reflection (ATR)-based evanescent waveabsorption method monitoring the localized surface plasmonresonance (LSPR). The developed surface was established as arefractive index sensor by monitoring the LSPR absorption peak ofgold nanoparticles. This nanoparticle-immobilized surface wasexplored to establish as a biosensing platform with target-specificimmunoglobulin (IgG) antibody−antigen interaction. The IgGconcentration-dependent variation of absorbance was correlated with the refractive index change. After successfully establishing thisATR configuration as an LSPR-based biosensor, the single-stranded DNA of the chilli leaf curl virus was detected using itscomplementary DNA sequence as a receptor. The limit of detection of this sensor was determined to be 1.0μg/mL for this targetviral DNA. This ATR absorption technique has enormous potential as an LSPR based nano-biosensor for the detection of otherbegomoviruses. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACS | en_US |
dc.subject | EEE | en_US |
dc.subject | Attenuated total reflection (ATR) | en_US |
dc.subject | Nano-biosensor | en_US |
dc.title | Detection of the Chilli Leaf Curl Virus Using an Attenuated Total Reflection-Mediated Localized Surface-Plasmon-Resonance-Based Optical Platform | en_US |
dc.type | Book | en_US |
Appears in Collections: | Department of Electrical and Electronics Engineering |
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