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Title: | Detection of the Chilli Leaf Curl Virus Using an Attenuated Total Reflection-Mediated Localized Surface-Plasmon-Resonance-Based Optical Platform |
Authors: | Rao, V. Ramgopal |
Keywords: | EEE Attenuated total reflection (ATR) Nano-biosensor |
Issue Date: | 2021 |
Publisher: | ACS |
Abstract: | The development of a nanoparticle-based opticalplatform has been presented as a biosensor for detecting target-specific plant virus DNA. The binding dynamics of goldnanoparticles has been studied on the amine-functionalized surfaceby the attenuated total reflection (ATR)-based evanescent waveabsorption method monitoring the localized surface plasmonresonance (LSPR). The developed surface was established as arefractive index sensor by monitoring the LSPR absorption peak ofgold nanoparticles. This nanoparticle-immobilized surface wasexplored to establish as a biosensing platform with target-specificimmunoglobulin (IgG) antibody−antigen interaction. The IgGconcentration-dependent variation of absorbance was correlated with the refractive index change. After successfully establishing thisATR configuration as an LSPR-based biosensor, the single-stranded DNA of the chilli leaf curl virus was detected using itscomplementary DNA sequence as a receptor. The limit of detection of this sensor was determined to be 1.0μg/mL for this targetviral DNA. This ATR absorption technique has enormous potential as an LSPR based nano-biosensor for the detection of otherbegomoviruses. |
URI: | https://pubs.acs.org/doi/10.1021/acsomega.1c01702 http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/12549 |
Appears in Collections: | Department of Electrical and Electronics Engineering |
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