Phytochemical screening, total phenol estimation, antioxidant activity of Blainville camellia leaf and stem successive extracts

Abstract

The aim of present work was to investigate antioxidant potential of different extracts of Blainvillea acmella leaf and stem. The successive extraction of individual plant part was carried out using solvents of different polarity viz. n-hexane, ethyl acetate, methanol and water. Preliminary phytochemical screening of all the extracts was done. The present total phenolic contents were estimated by Folin-Ciocalteu reagent method and expressed as µg/mg of gallic acid equivalent. The antioxidant potential and reducing power of all the prepared extracts were measured against DPPH, as compared to standard ascorbic acid, and BHA respectively. The result data indicate that the phenolic contents were higher in methanolic extracts of leaf (73.67±0.38 mg/g) followed by ethyl acetate (29.08±0.38 mg/g), aqueous (21.50±0.28 mg/g), and n-Hexane (9.29±0.38 mg/g); gallic acid equivalent. The similar pattern in stem part was also observed ie methanolic extracts (41.90±0.45 mg/g), ethyl acetate (21.92±0.28 mg/g), aqueous (15.13±0.18 mg/g), and n-Hexane (3.69±0.28 mg/g). The antioxidant capacity of methanolic extract of both the part ie leaf and stem was found to be maximum, as IC50 values were 226.49±0.16, 402.05±1.10 respectively. The reducing power was also highest in methanol extract of both parts. The result data conclude that the higher antioxidant as well as reducing power may be due to present phenolic contents