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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Taliyan, Rajeev | - |
dc.date.accessioned | 2023-12-13T06:51:57Z | - |
dc.date.available | 2023-12-13T06:51:57Z | - |
dc.date.issued | 2020 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlehtml/2020/ra/c9ra08121f | - |
dc.identifier.uri | http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/13393 | - |
dc.description.abstract | A reliable and sensitive ultra-performance liquid chromatography-tandem mass spectrometry-based method has been developed for the estimation of 4-hydroxyisoleucine (4-HI), a potent insulinotropic and hypolipidemic agent. The extraction of 4-HI from plasma was accomplished by the protein precipitation technique using L-isoleucine as an internal standard. The separation of analytes was achieved with a mobile phase consisting of acetonitrile and 0.1% formic acid in an isocratic flow system on a BEH Shield RP-18 column (150 mm × 2.1 mm, 1.7 μm). 4-HI and L-isoleucine were detected using an electrospray ionization (ESI) ion source, using multiple reaction monitoring (MRM) in positive ion mode. The precursor to product ion transitions of 4-HI and L-isoleucine were found at m/z values of 148.19 > 74.02 and 132.17 > 69.04, respectively. As per the guidelines for bioanalytical methods, all validation parameter results were within the acceptable range. The method exhibited a robust and reproducible linearity range of 1–5000 ng mL−1 with a coefficient of regression of 0.9999. The method was successfully applied for the estimation of pharmacokinetic parameters after oral administration of 4-HI (10 mg kg−1) in Wistar rats, by using Thoth Pro (version: 4.3) software. Herein, the two-compartment model was statistically fitted based on AIC and SBC values for evaluation of the pharmacokinetic parameters of 4-HI. Pharmacodynamic studies were also performed by measuring the levels of triglyceride and total cholesterol, and showed that the pharmacokinetic and pharmacodynamic data of 4-HI correlated with each other. | en_US |
dc.language.iso | en | en_US |
dc.publisher | RSC | en_US |
dc.subject | Pharmacy | en_US |
dc.subject | Pharmacodynamic | en_US |
dc.subject | Pharmacokinetics | en_US |
dc.subject | Chromatography-tandem | en_US |
dc.title | Pre-clinical pharmacokinetic and pharmacodynamic modelling study of 4-hydroxyisoleucine using validated ultra-performance liquid chromatography-tandem mass spectrometry | en_US |
dc.type | Article | en_US |
Appears in Collections: | Department of Pharmacy |
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