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dc.contributor.authorSidhu, Jagpreet Singh-
dc.date.accessioned2024-05-24T03:53:25Z-
dc.date.available2024-05-24T03:53:25Z-
dc.date.issued2023-05-
dc.identifier.urihttps://pubs.acs.org/doi/10.1021/acs.analchem.3c00099-
dc.identifier.urihttp://dspace.bits-pilani.ac.in:8080/jspui/xmlui/handle/123456789/14981-
dc.description.abstractEarly detection of Alzheimer’s disease (AD) is important for taking proper measures against AD pathogenesis. Acetylcholinesterase (AChE) is widely reported to be associated with the pathogenicity of AD. Here, employing the “acetylcholine-mimic” approach, we designed and synthesized a new class of naphthalimide (Naph)-based fluorogenic probes for specific detection of AChE and avoiding interference of butyrylcholinesterase (BuChE), the pseudocholinesterase. We investigated the action of the probes on Electrophorus electricus AChE, and the native human brain AChE that we expressed in Escherichia coli and purified in the active form for the first time. The probe Naph-3 exhibited a substantial fluorescence enhancement with AChE and majorly avoided BuChE. Naph-3 successfully crossed the cell membrane of the Neuro-2a cells and fluoresced upon reaction with endogenous AChE. We further established that the probe could be effectively used for screening AChE inhibitors. Our study provides a new avenue for the specific detection of AChE, which can be extended to the diagnosis of AChE-related complications.en_US
dc.language.isoenen_US
dc.publisherACSen_US
dc.subjectPharmacyen_US
dc.subjectCationsen_US
dc.subjectFluorescenceen_US
dc.subjectImaging probesen_US
dc.subjectPeptides and proteinsen_US
dc.subjectProbesen_US
dc.titleAcetylcholine Structure-Based Small Activatable Fluorogenic Probe for Specific Detection of Acetylcholinesteraseen_US
dc.typeArticleen_US
Appears in Collections:Department of Pharmacy

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