Simultaneous estimation of rapamycin, temozolomide and its metabolites using UPLC-ESI-MS/MS and its application to pharmacokinetics in C6-glioma bearing animals

Abstract

Therapeutic drug monitoring (TDM) plays a crucial role in the optimization of drug dosage profiles and establishing an optimal balance between toxicity and its effectiveness. Developing TDM methods for simultaneous estimation of multiple molecules with diverse physiochemical properties is quite complicated and difficult to achieve. Thus, hindering the quantitative estimation for TDM and in-depth analysis from bench to clinical settings. Therefore, the current study reports a bioanalytical method for the simultaneous determination of anticancer and immunosuppressant molecules (namely rapamycin, temozolomide, and its metabolites) in a single injection using whole blood as a biological matrix.