Priming with ACC-utilizing bacterium attenuated copper toxicity, improved oxidative stress tolerance, and increased phytoextraction capacity in wheat

Abstract

The major challenges for the plants growing in metal-contaminated soils are deficiency of nutrients, biomass reduction, and severe oxidative damages in the presence of heavy metals. In this regard, our aim was to overcome these challenges through the use of efficient microbial strains in metal-polluted soils and to assess its/their physiological and biochemical effects. In the current study, a copper (Cu)-resistant bacterium was isolated from the rhizospheric soil of ‘Ziziphus nummularia’ and evaluated for its ability to promote the wheat growth under the gradient stress of copper. Based on 16S rRNA gene sequencing, the isolate was identified as Pantoea sp. Among the plant growth promoting tests, the isolate showed the production of indole acetic acid, solubilization of inorganic phosphate, and ACC deaminase activity. Also, the isolate showed resistance to many heavy metals and antibiotics and increased the water-soluble copper in solution. The results of pot studies showed that bacterial application promoted various growth parameters of wheat plants and also enhanced the Cu uptake of wheat from the Cu-amended soil. The results showed that enhancement of Cu stress (100 to 300 mg kg−1) resulted in a decrease in various compatible solutes such as proline, total soluble sugars, and total protein content, and increase in the level of malondialdehyde (MDA), latter of which is the indicator of oxidative stress. Bacterial treatment markedly increased the proline, soluble sugar, total protein content, and decreased the MDA content under Cu stress. In addition, bacterial inoculation significantly alleviated the harmful effect of metal toxicity by decreasing the activation of ROS molecules including superoxide (O2 −) and hydrogen peroxide (H2O2). The activation of various antioxidative enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) was noted following bacterial inoculation under Cu stress. Therefore, the present study demonstrates the potential of the isolate Pantoea sp. ZNP-5 to improve the growth and phytoextraction of metal from the metal-polluted soil through the polyphasic mechanism of action.