Prediction of intrinsic disorder functions with DEPICTER2

Abstract

DEPICTER2 is a modern web server that provides convenient access to a broad selection of sequence-based predictions of intrinsic disorder and disorder functions. It incorporates six state-of-the-art methods that include ANCHOR2, DFLpred, DisoLipPred, DisoRDPbind, flDPnn, and MoRFCHiBi_Light, which predict disordered linkers and disordered regions that bind proteins, peptides, DNA, RNA, and lipids. DEPICTER2 facilitates selection of any combination of the six methods and batch predictions for multiple protein sequences. The prediction process is fully automated, performed on the server side, and does not require installation of any software. We describe and motivate selection of the six predictors, detail the prediction process, and explain how to interact with this web resource. We focus on the aspects related to the prediction of intrinsic disorder functions and provide a case study that illustrates how to interpret results produced by DEPICTER2.