Abstract:
A new, simple, specific and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence detector.
Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation method and 50 μ l of the standard solutions were injected into the column. The method was found to be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity and selectivity of the proposed method.