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Deep Ultraviolet Plasmonic Enhancement of Single Protein Autofluorescence in Zero-Mode Waveguides

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dc.contributor.author Patra, Satyajit
dc.date.accessioned 2024-04-24T08:54:51Z
dc.date.available 2024-04-24T08:54:51Z
dc.date.issued 2019-09
dc.identifier.uri https://pubs.acs.org/doi/full/10.1021/acs.nanolett.9b03137
dc.identifier.uri http://dspace.bits-pilani.ac.in:8080/jspui/xmlui/handle/123456789/14648
dc.description.abstract Single molecule detection provides detailed information about molecular structures and functions but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the sample production. Detecting a single protein with its natural UV autofluorescence is an attractive approach to avoid all the issues related to fluorescence labeling. However, the UV autofluorescence signal from a single protein is generally extremely weak. Here, we use aluminum plasmonics to enhance the tryptophan autofluorescence emission of single proteins in the UV range. Zero-mode waveguide nanoapertures enable the observation of the UV fluorescence of single label-free β-galactosidase proteins with increased brightness, microsecond transit times, and operation at micromolar concentrations. We demonstrate quantitative measurements of the local concentration, diffusion coefficient, and hydrodynamic radius of the label-free protein over a broad range of zero-mode waveguide diameters. Although the plasmonic fluorescence enhancement has generated a tremendous interest in the visible and near-infrared parts of the spectrum, this work pushes further the limits of plasmonic-enhanced single molecule detection into the UV range and constitutes a major step forward in our ability to interrogate single proteins in their native state at physiological concentrations. en_US
dc.language.iso en en_US
dc.publisher ACS en_US
dc.subject Chemistry en_US
dc.subject Fluorescence en_US
dc.subject Fluorescence spectroscopy en_US
dc.subject Molecules en_US
dc.subject Peptides and proteins en_US
dc.subject Plasmonics en_US
dc.title Deep Ultraviolet Plasmonic Enhancement of Single Protein Autofluorescence in Zero-Mode Waveguides en_US
dc.type Article en_US


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