Abstract:
The detection and discrimination of Cys amino acid from numerous other related biomolecules has great importance in clinical field for diagnosis of various diseases. Herein, to detect the Cys, we embedded the carbon dots (CDs), gold, and naphthalimide (L1) into a single ratiometric fluorescence sensor assembly. Sensor assembly works on the principle of FRET mechanism between CDs and naphthalimide when CDs and L1 adhered on gold nanoparticles surface. Gold metal was turned into solid support by in situ reduction of HAuCl4 in the presence of CDs and L1. When the assembly was excited at 360 nm, emission maxima at 568 nm corresponded to naphthalimide emission was emerged that signifies the existence of a FRET between the CDs and naphthalimide fluorophores. With the addition of Cys, the FRET mechanism eliminated and the change in the fluorescence emission at two different wavelengths (450 nm and 568 nm) was recorded. The endogenous images of Cys was recorded by collecting the fluorescence images of HeLa cells under fluorescence microscope and also applied for the assay of Cys in blood serum. Cytotoxicity studies of CDs and sensor assembly were evaluated by performing the MTT assay.