Abstract:
We have investigated the correlation between the concentration of geranyl acetate (GA) and acetyl CoA: geraniol acetyltransferase (GAAT) activity in palamarosa (Cymbopogon martini var. Motia) inflorescence and leaves at their different physiological stages. The results on GA concentration and the GAAT activity have been expressed on per gram fresh weight, per spikelet pair or leaf and per unit area of the phylloplane also incase of leaf. The percentage of geranyl acetate and geraniol in the volatile oil has also been considered. GA concentration was found to be highest in unopened floral spikelets and on the decline in fully open spikelets matching the trend of GAAT activity. Similarly, highest concentration of GA and maximum GAAT activity were found in leaves at mid-stage of development (stage II). The regression analysis curve between GA concentration (mg gFw−1) and GAAT activity (IU 10−3 gFw−1) gave an estimate of correlation coefficient (at 95% confidence) value of 0.79 for flowers and 0.92 for leaf. The results suggest that volatile ester (like geranyl acetate) synthesis in foliage and flowers of the aroma oil plant is controlled by the existent catalytic levels GAAT rather than the availability of geraniol. The study also indicates that the GAAT to be a good target to over-express for improvement of oil quality in terms of GA linked to fruit-fresh olfactory note of the oil.