| dc.description.abstract |
Bio-mitigation of CO2 utilizing prokaryotes and simultaneous extraction of valuable bio-molecule is fast gaining interest now-a-days. Present work discusses the thermodynamic assessment of CO2 bio-mitigation capability of Pseudomonas aeruginosa SSL-4 isolated from halo alkalophilic habitat in the absence of light. The maximum specific growth (μMax, h−1) of isolate was found to be 0.425 (±0.0025) and 0.34 (±0.0063) at 3% (w/v) salt concentration and 35 °C, respectively. The isolate was cultivated in the environment having initial CO2 (g) concentration of 17(±0.8) % (v/v) using Fe[II] as an energy source (0, 50 and 100 ppm) for evaluating CO2 fixing ability of microorganisms. The maximum CO2 removal efficiency of 92.37 (±2.46) % (v/v) was obtained at 100 ppm of Fe[II] concentration. The isolate has shown the maximum CO2 fixation rate () of 0.04 (±0.003) and 0.06 (±0.001) g/L/d at 50 and 100 ppm of Fe[II] concentration, respectively. FT-IR and GC–MS analysis of obtained leachate revealed the presence of fatty alcohols (C12–C28) and total product recovery (C12–C18) of 0.371 g per g of biomass. The thermodynamic assessment revealed the actual CO2 utilization efficiency of 41.16%. Thus, the isolated strain from extreme hyper saline environment has shown the potential for research dedicated to carbon capture and utilization. |
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