dc.contributor.author |
Deepa, P.R. |
|
dc.date.accessioned |
2021-09-17T04:44:03Z |
|
dc.date.available |
2021-09-17T04:44:03Z |
|
dc.date.issued |
2019 |
|
dc.identifier.uri |
https://link.springer.com/article/10.1007%2Fs00253-019-10274-7 |
|
dc.identifier.uri |
http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/2091 |
|
dc.description.abstract |
A novel fed-batch strategy based on carbon/nitrogen (C/N) ratio in a microbial co-culture production medium broth was carried out in a biocalorimeter for improved production of poly (3-hydroxybutyrate) (PHB). Shake flask study suggested that the C/N ratio of 10 increased the yield of PHB by 2.8 times. Online parameters monitored during the C/N ratio of 10 in biocalorimeter (BioRC1e) indicated that the heat profile was maintained in the fed-batch mode resulting in a PHB yield of 30.3 ± 1.5 g/L. The oxy-calorific heat yield coefficient during the fed-batch strategy was found to be 394.24 ± 18.71 kJ/O2 due to the oxidative metabolism of glucose. The reported heat-based model adapted for PHB concentration prediction in the present fed-batch mode. The heat-based model has a Nash-Sutcliffe efficiency of 0.9758 for PHB prediction. PHB obtained by fed-batch-mode was characterized using gas chromatography-mass spectrometry (GC-MS) for the monomer-acid analysis, Thermogravimetric analysis (TGA) for thermal stability of PHB, and Fourier transform infrared spectroscopy (FT-IR) for confirmation of functional groups. Here, we establish a favorable C/N ratio for achieving optimal PHB yield and a predictive heat-based model to monitor its production. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Springer |
en_US |
dc.subject |
Biology |
en_US |
dc.subject |
Biokinetics |
en_US |
dc.subject |
Microbial |
en_US |
dc.title |
Biokinetics of fed-batch production of poly (3-hydroxybutyrate) using microbial co-culture |
en_US |
dc.type |
Article |
en_US |