Understanding the Role of Adiponectin in Proliferative Diabetic Retinopathy Design and Development of Therapeutic Molecules

Abstract

Adiponectin (APN) is a protein hormone having anti-diabetic and anti-inflammatory properties.Its presence in ocular tissues has been postulated, to have escaped from circulation due to the newlinedysfunctional blood brain barrier (BRB) in retinal diseases. The purpose of this study is to newlineunderstand the origin of intraocular APN and its receptors, precisely measure vitreous APN in newlineproliferative diabetic retinopathy (PDR) and macular hole (MH), study its role in disease newlinepathogenesis of PDR and to unravel the therapeutic potential of APN. newlineAll human studies were done with approval from Institutional Review Board (IRB) and consent newlineof the participants. Tissues derived from donor eye balls were used for qPCR, immuno staining, newlinewestern blot analysis for localisation and expression studies. Vitreous samples were obtained newlinefrom patients who underwent vitrectomy surgery for PDR and MH and used for measurement of newlineAPN by ELISA. Vitreous amino acids were estimated by HPLC. Role of APN in the newlinepathogenesis of PDR was studied in retinal pericytes. Human retinal endothelial cells (HREC), newlinehuman choroidal endothelial cells (HCEC) and Human umbilical vein endothelial cells newline(HUVECs) were used for in vitro angiogenesis studies.