Department of Chemical Engineering

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Author: search.filters.author.Majumder, SubhajitSubject: search.filters.subject.Bioremediation

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    Estimation of Kinetic Parameters for Bioremediation of Cr(VI) from Wastewater Using Pseudomonas taiwanensis, an Isolated Strain from Enriched Mixed Culture
    (Taylor & Francis, 2014-08-08) Gupta, Suresh; Raghuvanshi, Smita; Majumder, Subhajit
    An aerobic mixed culture collected in the form of activated sludge was enriched for Cr(VI) reduction. An indigenous microorganism was isolated from the enriched aerobic mixed culture and identified as Pseudomonas taiwanensis. Bioremediation studies were carried out for treating Cr(VI)-contaminated wastewater using the indigenous microorganism. The kinetic studies were carried out for initial Cr(VI) concentrations ranging from 20 to 200 mg L−1. The maximum consumption of Cr(VI) obtained was 108.3 mg L−1 for an initial Cr(VI) concentration of 150 mg L−1 at a solution pH of 7.0. The effect of nutrient dosage and pH were studied to get their optimum values. The same isolated bacterial strain was also used to treat Cr(VI)-contaminated industrial wastewater collected from a local plating industry. Various growth kinetic models, such as Monod, Powell, Haldane, Luong, and Edward models, were fitted with the obtained experimental data. The obtained results for different growth kinetic models indicate that the growth kinetics of Pseudomonas taiwanensis for bioremediation of Cr(VI) can be better understood by the Luong model (R2 = .913). The rate kinetic analysis was performed using zero-order and three-half-order kinetic models. The three-half-order kinetic model was found to be suitable for the present bioremediation study.
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    A comprehensive study on the behavior of a novel bacterial strain Acinetobacter guillouiae for bioremediation of divalent copper
    (Springer, 2015-05-28) Gupta, Suresh; Raghuvanshi, Smita; Majumder, Subhajit
    Biological methods have been successfully used to mitigate heavy metal pollution problem in wastewater. The present study was aimed towards isolation of a novel indigenous bacterial strain, Acinetobacter guillouiae from activated sludge and its subsequent application in remediation of copper (Cu2+) from aqueous solution. Kinetic study of bioremediation was performed for initial Cu2+ concentrations ranging from 40 to 150 mg L−1. Optimum values of nutrient dosage, pH, macronutrients [Nitrogen (N)–Phosphorus (P)–Potassium (K)] dosage, aerobic and facultative anaerobic conditions, temperature, and inoculum volume were determined by conducting separate batch bioremediation studies at 80 mg L−1 initial concentration of Cu2+. Kinetic study showed that A. guillouiae removed 98.7 % Cu2+ for 80 mg L−1 initial concentration of Cu2+ after 16 h at an optimum solution pH of 7.0. Results also revealed that A. guillouiae showed maximum growth at double the standard composition of N, P and standard composition of K in nutrient dosage. Experimental data obtained in present study were utilized to validate different growth kinetic models such as Monod, Powell, Haldane, Luong, and Edwards. Growth kinetics of A. guillouiae was better understood by Luong model (R 2 = 0.97). Higher values of coefficient of determination (R 2 = 0.97–0.99) confirmed the suitability of the three-half-order kinetic model for representing the Cu2+ bioremediation. A. guillouiae showed a robust removal mechanism for the bioremediation of Cu2+.