Department of Biological Sciences

Permanent URI for this collectionhttp://localhost:4000/handle/123456789/1922

Browse

Filters

2010 - 201912020 - 20251

Settings

Author: search.filters.author.Joshi, MukulSubject: search.filters.subject.Abiotic stress

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Mechanisms of sensing abiotic stress responses in plants
    (Elsevier, 2025) Joshi, Mukul
    Plants encounter various environmental stresses, and they need unique strategies to adapt to such adverse conditions. This chapter represents the mechanisms of sensing abiotic stresses and responses in plants. This includes the stress signal reception, sensing, and transduction via different factors into intracellular signaling, further inducing stress-responsive genes and proteins. After receiving the stress on the cell surface and sensing it by primary messengers, intracellular Ca2+ ions are major messengers that increase during most stress-induced signal transduction pathways. The induced Ca2+ initiates different pathways for different abiotic stresses and downstream cellular processes, many of which are common to various stresses and result in stress-specific physiological and developmental responses. Significant progress has been made in understanding the early to downstream events in abiotic stress signaling in plants, which is reviewed and documented in this chapter.
  • No Thumbnail Available
    Item
    Cloning and characterization of the Salicornia brachiata Na+/H+ antiporter gene SbNHX1 and its expression by abiotic stress
    (Springer, 2010-09) Joshi, Mukul
    Salinity causes multifarious adverse effects to plants. Plants response to salt stress involves numerous processes that function in coordination to alleviate both cellular hyperosmolarity and ion disequilibrium. A Na+/H+ antiporter NHX1 gene has been isolated from a halophytic plant Salicornia brachiata in this study. Predicted amino acid sequence similarity, protein topology and the presence of functional domains conserved in SbNHX1 classify it as a plant vacuolar NHX gene. The SbNHX1 cDNA has an open reading frame of 1,683 bp, encoding a polypeptide of 560 amino acid residues with an estimated molecular mass 62.44 kDa. The SbNHX1 shows high amino acid similarity with other halophytic NHX gene and belongs to Class-I type NHXs. TMpred suggests that SbNHX1 contains 11 strong transmembrane (TM). Real time PCR analysis revealed that SbNHX1 transcript expresses maximum at 0.5 M. Transcript increases gradually by increasing the treatment duration at 0.5 M NaCl, however, maximum expression was observed at 48 h. The overexpression of SbNHX1 gene in tobacco plant showed NaCl tolerance. This study shows that SbNHX1 is a potential gene for salt tolerance, and can be used in future for developing salt tolerant crops.