Department of Biological Sciences

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Author: search.filters.author.Verma, Sanjay KumarEnd date: 2019

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Now showing 1 - 10 of 41
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    Direct saponification of wet microalgae by methanolic potassium hydroxide using acetone as co-solvent
    (Elsevier, 2019-02) Verma, Sanjay Kumar
    The fatty acids of microalgae are promising source of biodiesel and omega-3 fatty acids. The objective of this study is extraction of fatty acids from wet Dunaliela salina by direct saponification using methanolic potassium hydroxide and a suitable co-solvent. The study identified acetone as an efficient co-solvent for saponification of wet microalgal biomass by methanolic potassium hydroxide. The presence of acetone in methanolic KOH saponification produced 24% more fatty acid than its absence. Further optimization of concentration of acetone (80% v/v), concentration of potassium hydroxide (0.1% v/v) and solvent-dry biomass ratio (75:1) resulted in a maximum yield of 98% fatty acid. The proposed acetone assisted saponification of wet microalgal biomass could serve as an energy efficient route for fatty acid production since the reaction happens under room temperature and normal atmospheric pressure without any cell lysis or drying the biomass.
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    Molecular Databases
    (EMI, 2001) Verma, Sanjay Kumar
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    Bioremediation of cadmium by metal resistant strain of Cyanobacteria
    (Scientific Publisher, 2002) Verma, Sanjay Kumar
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    Secondary metabolite production of cyanobacteria
    (IK Books, 2009) Verma, Sanjay Kumar
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    Impact of the Intellectual Property System on Economic Growth
    (WIPO, 2010) Verma, Sanjay Kumar; Rao, N.V.M.
    There has been a lot of controversy on the role of intellectual property protection (IPP) regime especially the patent system in fostering innovation,technology and industrial development of a country. IPP is expected to encourage innovation by rewarding the inventor. Strong IPP regime may also inhibit diffusion of knowledge and even technology development in the countries that are technology followers. Countries have fine-tuned their IPP regimes as per their developmental requirements. Against this backdrop, the on-going attempt to harmonize and strengthen the IPP regimes worldwide, as a part of the TRIPs Agreement, is widely seen to be adversely affecting the technological activity in developing countries by choking the knowledge spillovers besides implications for the access and affordability to lifesaving drugs by the poor.
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    Remediation of Arsenic Toxicity
    (Taylor & Francis, 2016) Verma, Sanjay Kumar; Chowdhury, Rajdeep
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    Heavy metal Uptake in the Cynobacterium Nostoc calcicola.
    (IBS, 1988) Verma, Sanjay Kumar
    The literature on the inhibitory effect of copper on growth (Les &Walker, 1984), photosynthesis (Shiol et al, 1978) and nitrogen fixation (Rai & Raizada, 1985) in algae and higher plants is impressive. Heavy metals inhibit the uptake and assimilation of nutrients (Singh & Yadava, 1983, 1984). Mechanisms governing the metal uptake process in microorganisms (Bhattacharjee, 1986) and in cyanobacteria (Singh & Yadava, 1985) are investigated. The possibility of involvement of carrier (s) and energy in the metal transport across the cell membranes has been suggested (Veltrup, 1977). The toxicity of heavy metal to algae (Hargreaves & Whitton, 1976 a,b; Singh & were diluted to S hli with triple glass distilled water. A furher Pandey 1981) is influenced by environmental factors like pH, light and complexans. However, there have been only a few studies on the effect of environmental factors on copper uptake in cyanobacteria. The present investigation reports on the copper uptake kinetics and the role of various factors on Nostoc calcicola.
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    Copper uptake by free and immobilized cyanobacterium
    (OUP, 1989) Verma, Sanjay Kumar
    Copper uptake in free and immobilized cells of the cyanobacterium Nostoc calcicola has been examined. The immobilized cells invariably maintained a higher profile of Cu intake rate (12.7 nmol mg−1 protein min−1) over the free cells (6.0 nmol mg−1 protein min−1). The total Cu uptake in immobilized cells was almost two and a half-times more than their free cell counterpart under identical experimental conditions. Also, the immobilized cells showed a stronger positive correlation between Cu adsorption and uptake. The results have been discussed in terms of improved metabolic efficiency of immobilized cells.
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    Factors regulating copper uptake in a cyanobacterium
    (Springer, 1990) Verma, Sanjay Kumar
    Copper uptake in the diazotrophic cyanobacteriumNostoc calcicola was found to be typically biphasic, comprising rapid binding of the cations to the cell wall (during the first 10 min) followed by the subsequent metabolism-dependent intracellular uptake for at least 1 h, with a curvilinear kinetics saturating at 40 µM (Km 25.0 µM, Vmax 3.0 nmol Cu mg−1 protein min−1). The cellular Cu uptake was light- and ATP-dependent, and the addition of 3(3,4-dichlorophenyl)-1,1-dimethylurea or exogenous ATP proved that the energy to drive Cu transport was derived mainly through PS II reactions. The application of metabolic inhibitors and uncouplers like carbonylcyanidep-nitrofluoromethoxylphenyl hydrazone, N,N′-dicyclohexycarbodiimide, azide, and p-chloromercuribenzoate revealed that -SH group(s), proton gradient across the cell membrane, and ATP hydrolysis were involved in the transmembrane movement of Cu inN. calcicola. While monothiol (2-mercaptoethanol) caused a twofold reduction in Cu uptake rate, dithiol (dithiothreitol) contributed towards a further drop in the cation uptake rate.