Department of Biological Sciences
Permanent URI for this collectionhttp://localhost:4000/handle/123456789/1922
Browse
4 results
Search Results
Item Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells(Tech Science Press, 2021-09) Dubey, Uma S.Sorafenib, a multikinase inhibitor used for the treatment of hepatocellular carcinoma, is limited by its low oral bioavailability. To overcome this drawback, we have developed novel camel milk casein-derived nanoparticles as a drug delivery system. Camel milk casein is not only biocompatible on oral administration but is actually a dietary protein of pharmaceutical relevance. Casein is used because of its amphiphilic nature, self-assembling property, ability to show sustained release, and capability of encapsulating both hydrophilic and hydrophobic drugs. In this study, camel milk casein nanoparticles loaded with sorafenib were developed and characterized. Characterization of casein nanoparticles was done by dynamic light scattering (DLS), zeta potential analysis, scanning light microscopy (SEM), and FTIR. The drug content in nanoparticle and drug-protein binding studies were conducted by UV spectroscopy. The cytotoxicity and cellular uptake efficiency studies were performed in HepG2 cell lines. It was observed that the cytotoxic effect of sorafenib loaded camel milk casein nanoparticles was more than free sorafenib in HepG2 cells. This work suggests camel milk casein as a suitable drug delivery molecule for sorafenib. In the future, it may also be used in enhancing the efficacy and specific distribution of other water-insoluble anticancer drugs.Item Medicinal potential of Camel milk lactoferrin(Intecopen, 2022) Dubey, Uma S.Camel milk is a rich source of protein with well-recognized medicinal properties to treat various diseases. The objective of this work is to understand the role of camel milk lactoferrin in immunomodulation and in disease treatment. It has been found that camel milk lactoferrin is a very suitable nutraceutical agent by virtue of its bioac- tivity, immuno-compatibility, and safety. It can be used for the treatment of infec- tious, metabolic, and neurodegenerative diseases, besides cancer. It is a cost-effective biomolecule that also has high relative abundance and bioavailability.Item Studies on cytotoxic activity of Camel milk whey protein as a nutraceutical against HeLa cells(Camel Publishing House, 2023) Dubey, Uma S.Camel milk is an adapted dietary supplement with multiple antimicrobial and immuno-stimulatory properties. Diabetes, infant diarrhoea, hepatitis, allergy, lactose intolerance, and alcohol- induced liver damage have been treated with it (Galil et al, 2016). Numerous immunologically essential molecules, such as lysozymes, lactoferrin, lactoperoxidase, serum albumin, acidic whey protein, peptidoglycan recognition protein, and small peptides, contribute to its health benefits (Dubey et al, 2016). Recently, the anti-microbial and antioxidant properties of camel milk and its role as an anti-cancer and anti-hepatitis agent has been demonstrated (Khan et al, 2021). Camel milk also has the normal isotypes of antibodies shared with other mammalian species. Actually not only camel’s milk but even its urine is among such natural products enriched with molecules that are safe to humans and endowed with profound anti-cancer properties (Alebie et al, 2017). Aryl hydrocarbon receptor (AhR) is an endogenous transcription factor with known preventative and therapeutic benefits for patients with cancer associated with organs like the liver, breast, prostate, etc. (Xie et al, 2012; Richmond et al, 2014). Aberrant AhR expression is involved in carcinogenesis (Korzeniewski et al, 2010).Item Isolation and characterization of bioactive lactoferrin from camel milk by novel pH-dependent method for large scale production(Elsevier, 2022-12) Dubey, Uma S.The present article exemplifies a novel method to isolate highly purified bioactive lactoferrin from camel milk. Cytotoxicity of lactoferrin against the Hela cells was used to evaluate its bioactivity. SDS-PAGE and LC-MS analysis was done for its identification and characterization. The purified camel milk lactoferrin was found to be 708 amino acids in length with a molecular weight of 77.3 kDa and a pI value of 8.24. This pH-dependent isolation procedure ensures the retention of bioactive lactoferrin from camel milk. The importance of the present work lies in its simplicity and scalability for manufacturing bioactive lactoferrin at an industrial level.