Department of Biological Sciences

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Subject: search.filters.subject.Bacillus

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    Characterization and Optimization of Alkaline Protease Production from Bacillus licheniformis HSW-16 Isolated from Sambhar Salt Lake
    (IJASB, 2015) Jha, Prabhat N.
    Halophilic microorganisms are recognized as potential source of secondary metabolites including enzymes and drugs with wide agricultural and industrial applications. In the present study protease producing halotolerant bacterium Bacillus licheniformis HSW-16 was isolated from hypersaline Sambhar lake, Rajasthan India. Protease production was performed by using azocasein as substrate. Confirmation of protease production was also done by amplification of alkaline protease gene and sequencing. The various nutritional factors such as carbon and nitrogen source and other physiological parameters like pH, temperature, incubation time and agitation speed were optimized for optimum protease production. The enzyme was active in pH range 7-10, temperature 25 °C-40 °C and salt concentration of 1.5M. The characteristics demonstrated by this isolate showed that it could be used as a potential source of enzyme.
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    GC-MS ANALYSIS OF CHANGE IN FATTY ACID COMPOSITION OF HALOBACTERIUM BACILLUS LICHENIFORMIS HSW-16 UNDER VARYING SALINITY CONDITION
    (NITRA, 2015) Jha, Prabhat N.; Raghuvanshi, Smita
    The salt concentration have a pronounced effect on fatty acid composition, therefore the adaption of fatty acid composition of a halophilic bacterium Bacillus licheniformis HSW-16, grown at different concentration of NaCl (0 mM, 150 mM, 175 mM, 200 mM) was studied. The common fatty acid observed at various salt concentration were tetratriacontane, triacontane, tetracosane and pentacosane etc..As the salinity increases from 150 mM to 200 mM, the bacterium started to accumulating the long chain alkanes and fatty alcohol, suggesting the adaption of bacterium through accumulation varying fatty acids for counteracting the increased salinity and maintaining its osmotic balance in the changing environment