BITS Faculty Publications
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Item Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor(MDPI, 2021-09) Gupta, Suresh; Raghuvanshi, Smita; Verma, Sanjay KumarToday’s society is faced with many upfront challenges such as the energy crisis, water pollution, air pollution, and global warming. The greenhouse gases (GHGs) responsible for global warming include carbon dioxide (CO2), methane (CH4), nitrous oxide (NOx), water vapor (H2O), and fluorinated gases. A fraction of the increased emissions of CO2 in the atmosphere is due to agricultural and municipal solid waste (MSW) management systems. There is a need for a sustainable solution which can degrade the pollutants and provide a technology-based solution. Hence, the present work deals with the custom design of a loop photobioreactor with 34 L of total volume used to handle different inlet CO2 concentrations (0.03%, 5%, and 10% (v/v)). The obtained values of biomass productivity and CO2 fixation rate include 0.185 ± 0.004 g L−1 d−1 and 0.333 ± 0.004 g L−1 d−1, respectively, at 10% (v/v) CO2 concentration and 0.084 ± 0.003 g L−1 d−1 and 0.155 ± 0.003 g L−1 d−1, respectively, at 5% (v/v) CO2 concentration. The biochemical compositions, such as carbohydrate, proteins, and lipid content, were estimated in the algal biomass produced from CO2 mitigation studies. The maximum carbohydrate, proteins, and lipid content were obtained as 20.7 ± 2.4%, 32.2 ± 2.5%, and 42 ± 1.0%, respectively, at 10% (v/v) CO2 concentration. Chlorophyll (Chl) a and b were determined in algal biomass as an algal physiological response. The results obtained in the present study are compared with the previous studies reported in the literature, which indicated the feasibility of the scale-up of the process for the source reduction of CO2 generated from waste management systems without significant change in productivity. The present work emphasizes the cross-disciplinary approach for the development of bio-mitigation of CO2 in the loop photobioreactor.Item Deducing the Bio-Perspective Capabilities of Fe(II) Oxidizing Bacterium Isolated from Extreme Environment(BAB, 2015) Jha, Prabhat N.; Mishra, Somesh; Raghuvanshi, Smita; Gupta, SureshRigorous utilization of chemical fertilizers, monoculture and irrigation with surface saline water is globally deteriorating the quality of surface soil. In present work, the halo and alkalo tolarent bacterium strain isolated from extreme environment, was explored for producing plant growth hormones, that could be used for salt stress up gradation of the crops. The 16S rRNA gene sequencing was used for analysis of salt tolerant bacterium. The bacterium was identified as Pseudomonas aeruginosa KP163922. The Pseudomonas aeruginosa KP 163922 showed plant growth promotion traits, production of industrially important enzymes (amylase, protease and cellulase) and tolerance to more than 4% NaCl. The antagonistic test reveals that, the growth of pathogenic gram negative bacterium E. coli and Pseudomonas putida was repressed by Pseudomonas aeruginosa KP163922Item GC-MS ANALYSIS OF CHANGE IN FATTY ACID COMPOSITION OF HALOBACTERIUM BACILLUS LICHENIFORMIS HSW-16 UNDER VARYING SALINITY CONDITION(NITRA, 2015) Jha, Prabhat N.; Raghuvanshi, SmitaThe salt concentration have a pronounced effect on fatty acid composition, therefore the adaption of fatty acid composition of a halophilic bacterium Bacillus licheniformis HSW-16, grown at different concentration of NaCl (0 mM, 150 mM, 175 mM, 200 mM) was studied. The common fatty acid observed at various salt concentration were tetratriacontane, triacontane, tetracosane and pentacosane etc..As the salinity increases from 150 mM to 200 mM, the bacterium started to accumulating the long chain alkanes and fatty alcohol, suggesting the adaption of bacterium through accumulation varying fatty acids for counteracting the increased salinity and maintaining its osmotic balance in the changing environmentItem CO2 sequestration potential of halo-tolerant bacterium Pseudomonas aeruginosa SSL-4 and its application for recovery of fatty alcohols(Elsiever, 2017-10) Jha, Prabhat N.; Mishra, Somesh; Gupta, Suresh; Raghuvanshi, SmitaBio-mitigation of CO2 utilizing prokaryotes and simultaneous extraction of valuable bio-molecule is fast gaining interest now-a-days. Present work discusses the thermodynamic assessment of CO2 bio-mitigation capability of Pseudomonas aeruginosa SSL-4 isolated from halo alkalophilic habitat in the absence of light. The maximum specific growth (μMax, h−1) of isolate was found to be 0.425 (±0.0025) and 0.34 (±0.0063) at 3% (w/v) salt concentration and 35 °C, respectively. The isolate was cultivated in the environment having initial CO2 (g) concentration of 17(±0.8) % (v/v) using Fe[II] as an energy source (0, 50 and 100 ppm) for evaluating CO2 fixing ability of microorganisms. The maximum CO2 removal efficiency of 92.37 (±2.46) % (v/v) was obtained at 100 ppm of Fe[II] concentration. The isolate has shown the maximum CO2 fixation rate () of 0.04 (±0.003) and 0.06 (±0.001) g/L/d at 50 and 100 ppm of Fe[II] concentration, respectively. FT-IR and GC–MS analysis of obtained leachate revealed the presence of fatty alcohols (C12–C28) and total product recovery (C12–C18) of 0.371 g per g of biomass. The thermodynamic assessment revealed the actual CO2 utilization efficiency of 41.16%. Thus, the isolated strain from extreme hyper saline environment has shown the potential for research dedicated to carbon capture and utilization.Item Effect of inoculation of zinc-resistant bacterium Enterobacter ludwigii CDP-14 on growth, biochemical parameters and zinc uptake in wheat (Triticum aestivum L.) plant(Elsiever, 2018-03) Jha, Prabhat N.; Mishra, Somesh; Raghuvanshi, SmitaA metal resistant bacterium was isolated from the rhizosphere of Kair ‘Capparis decidua’ and screened for its phytoextraction ability under gradient metal stress conditions. Based on 16S rDNA analysis, the strain was identified as Enterobacter ludwigii. Among the plant growth promoting traits, isolate showed the ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, production of indole-3-acetic acid in tryptophan supplemented medium and solubilize the inorganic phosphate. The isolate was resistant to heavy metals like zinc (Zn), nickel (Ni), copper (Cu), and cadmium (Cd). The fatty acid adaptation of isolate growing at different concentration of Zn (100–300 mg kg−1) was also studied, which indicated that metal concentration strongly influenced the fatty acid composition of bacterium, particularly by increasing the unsaturated fatty acids. Furthermore, inoculation with the test isolate was found to significantly (p < 0.01) increase the various growth parameters of wheat plants and also improve the photosynthetic pigments. In addition, inoculation with isolate resulted in significant (p < 0.01) increase in the Zn content in wheat plant under metal stress. Moreover, bacterial application significantly (p < 0.01) increased the various compatible solutes such as proline content (30–65%), total soluble sugar (9–49%), and decreased the malondialdehyde (MDA) content (38–47%) as compared to control, illustrating its protective effect under metal induced oxidative stress. Inoculation with test isolate also increased the total protein content in range of 16–52%. Our work revealed that metal resistant plant growth promoting rhizobacterium could be exploited as microbial mediated phytoremediation of metal polluted soils.