BITS Faculty Publications
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Item Secondary metabolite production of cyanobacteria(IK Books, 2009) Verma, Sanjay KumarItem Nostoc calcicola Immobilized in Silica-coated Calcium Alginate and Silica Gel for Applications in Heavy Metal Biosorption(Springer, 2009) Verma, Sanjay KumarThe present study reports the preparation and characterization of silica-based immobilization matrices for the purpose of metal accumulation using immobilized cyanobacterium Nostoc calcicola. Silica gel was prepared using aqueous sodium silicate and colloidal silica. Calcium alginate (CAG) beads were coated with silica using sodium silicate solutions. Microscopy observations and TTC tests confirmed that the immobilized cells were intact and viable. Ultrastructural studies with electron microscopy revealed a membrane thickness of approximately 10 μm around the CAG and the silica gel to be of mesoporous nature. BET surface area of silica gel-immobilized N. calcicola was 160 m2 g−1. The porous volume and average pore diameter were 0.40 cm3 g−1 and ca. 100 Å, respectively, as calculated using the BJH model. Studies on silica-coated calcium alginate immobilized cells showed that these were superior to the uncoated CAG beads in terms of mechanical strength and metal accumulation. The silica matrices were found to be stable for repeated cycles of metal removal and with commonly used eluants for desorption processes. These matrices have potential applications in immobilization of industrially important biocatalysts.Item Application of Direct Analysis in Real Time Mass Spectrometry (DART-MS) for Identification of an Epiphytic Cyanobacterium, Nostoc Sp.(Taylor & Francis, 2012-05-12) Verma, Sanjay KumarCyanobacteria, a diverse group of bacteria are identified entirely on the basis of morphological characters, which can frequently lead to incorrect results; therefore, the present study reports a new approach for identification of an organism based on DART technique. Direct Analysis in Real Time Mass Spectrometry (DART-MS) was used to identify a cyanobacterium, isolated from the leaf surface, on the basis of characteristic chemical compounds present in the strain. A chemical fingerprint was generated and peaks obtained were found to be similar to the masses of the compounds reported for Nostoc spItem Application of Mutant Strains of Cyanobacteria for Cd2+ Removal(Springer, 2002-11) Verma, Sanjay KumarThe current practice for the removal of soluble metal ions present in the industrial effluents includes simple chemical precipitation, ion exchange, ion specificity, narrow range of pH and poor settling colloidal properties may limit the effectiveness..