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Item Role of Iron in Pathogenesis of Chlamydia trachomatis Infection in vitro(BITS PILANI, Pilani campus, 2010) Vardhan, HarshItem Molecular Diagnosis and Pathogenesis of Chlamydia pneumoniae in Coronary Artery Disease Patients(BITS PILANI, Pilani campus, 2010) Jha, Hem ChandraItem Study on the interaction between the rhizobacterium, Pseudomonas putida AKMP7, and, Arabidopsis thaliana, under drought conditions: case of conditional pathogenesis(BITS PILANI, Hyderabad campus, 2024) Raja Gopalan, N SItem Studies on the Role of Chlamydia trachomatis Infection in the Pathogenesis of Reactive Arthritis/ Undifferentiated Spondyloarthropathy(BITS Pilani, 2018-07-18) Kumar, PraveenReactive Arthritis/ undifferentiated spondyloarthropathy due to Chlamydia newlinetrachomatis is largely asymptomatic hence get unnoticed further, it causes chronic newlineinfection in major joints of male and female patients. Role of Chlamydia trachomatis in newlinethe pathogenesis in ReA/ uSpA patients was studied by estimating the frequency of newlineinfection. Intra-articular infection for either C. trachomatis MOMP/ plasmid was found in newline24.4% (11/45) ReA/ uSpA patients by either snPCR/ nPCR; plasmid and MOMP were newlinefound in 11/45 (24.4%) and 7/45 (15.5%) patients, respectively. 28.5% (6/21) ReA and newline20.8% (5/24) uSpA patients were found positive for either MOMP/ plasmid gene of C. newlinetrachomatis. The plasmid gene was also detected by PCR (conventional) in the urine of newline17.3% (6/35) uSpA patients without effusions. Cytological detection by DFA showed the newlinepresence of C. trachomatis EBs in the SF of 31.1% (14/45) ReA/ uSpA patients; while in newlinethe urine of uSpA patients without effusions, 13.3% (6/45) were C. trachomatis-positive. newlineAlthough anti-chlamydial antibodies (IgM, IgG, IgA) were detected in SF/ serum of ReA/ newlineuSpA patients, however, only anti-C. trachomatis IgA antibodies showed diagnostic newlineimportance (SF- 31.1%; 14/45 and serum 15.5%; 7/45). It was concluded that nPCR newlineshould be the method of choice for diagnosing intra-articular C. trachomatis DNA in SF newlineof chronic ReA/ uSpA patients with effusion. DFA for an initial rapid detection of an newlineacute episode of infection followed by the more confirmatory molecular diagnostic newlinemethod. Overall, the SF was found to be a more conclusive clinical specimen in both newlineacute and chronically C. trachomatis-infected ReA/ uSpA patients with effusion in terms newlineof molecular diagnosis by nPCR, C. trachomatis MOMP antigen detection and antichlamydial antibody response.Item Role of Iron in Pathogenesis of Chlamydia trachomatis Infection in vitro(BITS Pilani, 2010) VARDHAN, HARSHChlamydia trachomatis (CT) is a gram-negative obligate intracellular parasite responsible for newlinemyriad of diseases including trachoma, pelvic inflammatory diseases and infertility. newlineChlamydial biphasic developmental cycle is closely interwoven with host metabolic and newlineregulatory pathways for its requirements. Virtually all cells and organisms utilize iron as a newlinecofactor in a multitude of biochemical activities. Iron, as an essential nutrient and potential newlinetoxin poses an exquisite regulatory problem in biology and medicine. Availability of iron newlinedetermines the course and disease outcome of chlamydial infection. In the present study CThost newlineinteraction was assessed in HeLa 229 cells under iron depleted and supplemented newlineconditions. newlineIt was observed that at critical concentration of 25-50uM deferoxamine (DFO), CT infection newlinelead to greater sustenance of cells and delayed chlamydial developmental cycle as observed newlinein cell viability assay and direct fluorescence assays. Further irrespective of iron depletion newlineand supplementation CT infected HeLa cells showed decrease in surface expression of newlinetransferrin receptor (TfR) (flow cytometry), however level of ferritin heavy chain (FHC) newlineincreased as observed in immunoblotting. Intracellular ROS and mitochondrial membrane newlinepotential was analysed using DCFH-DA and JC-1 probes respectively. Level of ROS was newlinedampened, moreover mitochondrial membrane potential remained stable in iron depleted and newlinesupplemented conditions in CT infected cells. Increase in level of anti-apoptotic Bcl-2, Trx, newlinePPAR-and#947; and decrease in pro-apoptotic cytoplasmic cytochrome c , active caspase-3/8/9 and newlineBax was observed in CT infected cells. These results suggested that critical concentration newline(25-50uM) of DFO was synergistic to anti-apoptotic effect of CT.