Transcriptomic profiling of desert tree Prosopis cineraria under heat stress reveals potential role of multiple gene families in its high thermotolerance

Abstract

The static nature of plants restrains their potential to evade heat stress and requires them to withstand stress through inherent defence abilities. Prosopis cineraria is a leguminous phreatophyte distributed across arid and semi-arid regions of India and can tolerate very high temperatures due to its adaptive physiological and biochemical mechanisms. Therefore, P. cineraria represents a repository of genes for abiotic stress tolerance. Two-months-old P. cineraria plants were subjected to heat stress at two different temperature regimes and transcriptome sequencing was performed to identify differentially expressed genes (DEGs). A total of 1151 and 1562 DEGs were observed in response to 45℃ and 55℃ heat stress compared to control, respectively, indicating that 55℃ treatment has a pronounced effect on P. cineraria. The transcriptomic data highlighted the potential role of multiple gene families and their interactions for high thermotolerance of P. cineraria. The expression of a few representative heat stress-responsive genes was validated with real-time qPCR. The in-depth bioinformatic analysis provided the detailed transcriptome profiling, supported by its validation, and new insights for important abiotic stress-related genes from thermotolerant P. cineraria, which can be used for crop improvement.