Drosophila Adult Eye Model to Teach Scanning Electron Microscopy in an Undergraduate Cell Biology Laboratory

Abstract

We have devised an undergraduate laboratory exercise to study tissue morphology using fruit fly, Drosophila melanogaster, as the model organism. Drosophila can be reared in a cost effective manner in a short period of time. This experiment was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of Scanning Electron Microscopy (SEM) technique to study the morphology of adult eye of Drosophila. The adult eye of Drosophila is a compound eye, which comprises of 800 unit eyes, and serves as an excellent model for SEM studies. We used flies that were mutant for Lobe (L), eyeless (ey), and pannier (pnr) for our studies. The mutant flies exhibit different morphologies of the adult eye.