Molecular identification and characterization of rhizospheric bacteria for plant growth promoting ability

Abstract

The objective of this study was to isolate and characterize a rhizospheric bacterium from Sorgastrum nutans, growing around the desert region of Rajasthan (India). Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Isolated strain was tested for various PGP traits like 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, phosphate solubilization, indole acetic acid production, production of siderophore, nitrogen fixation and ammonia production. Bio-control ability of isolate was screened by antagonistic activity against certain fungal/bacterial pathogens as well as Hydrogen cyanide HCN production. Isolated test organism was also biochemically characterized. Further identification of isolate was performed by PCR based 16S rRNA gene sequencing. Moreover evaluation of the isolate SNP-18 exhibiting multiple plant growth promoting (PGP) traits on soil/plant system is on-going to uncover their efficacy as effective PGPR.