Effect of feeding strategies on inulinase production analyzed in a biocalorimeter
| dc.contributor.author | Deepa, P.R. | |
| dc.date.accessioned | 2021-09-17T04:44:49Z | |
| dc.date.available | 2021-09-17T04:44:49Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Kluyveromyces marxianus exoinulinase production in shaker and bio reaction calorimeter has been studied. Biocalorimetric studies revealed enhanced inulinase production due to the linear and exponential feeding of sucrose. Linear fed-batch inulinase production (355 IU/mL) was seventeen-fold greater than batch mode (20.98 IU/mL). Further controlling the specific growth rate by exponential feeding of sucrose gave highest inulinase activity of 728 IU/mL. Exponential feeding strategy yielded highest inulinase activity in the synthetic medium with a reduction in batch time to half that of the linear feeding. This has been substantiated by metabolic and physiological aspects of inulinase production. A Michaelis-Menten type empirical model was developed for fed-batch that can be adopted for controlling specific growth rate based on metabolic heat responses, towards achieving maximum enzyme production and release. | en_US |
| dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1359511316300423?via%3Dihub | |
| dc.identifier.uri | http://dspace.bits-pilani.ac.in:8080/xmlui/handle/123456789/2099 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsiever | en_US |
| dc.subject | Biology | en_US |
| dc.subject | Kluyveromyces marxianus | en_US |
| dc.subject | Biocalorimetry | en_US |
| dc.subject | Inulinase | en_US |
| dc.subject | Fed-batch | en_US |
| dc.title | Effect of feeding strategies on inulinase production analyzed in a biocalorimeter | en_US |
| dc.type | Article | en_US |
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