Evaluation of Antioxidant Potential of Barleria prionitis Leaf and Stem

Abstract

The aim of the present work was to investigate the antioxidant potential of different extracts of Barleria prionitis leaf and stem. The successive extraction of individual plant part was carried out using solvents of different polarity viz. n-hexane, ethyl acetate, methanol and water. The preliminary Phytochemical screening of all the extracts was done. The present total phenolic contents were estimated by Folin- Ciocalteu reagent method and expressed as μg/mg of gallic acid equivalent. The antioxidant potential and reducing power of all the prepared extracts were measured against DPPH as compared to standard ascorbic acid, and BHA respectively. The result data indicated that the phenolic contents were higher in methanolic extracts of leaf (103.51±0.38 mg/g) followed by ethyl acetate (52.91±0.28 mg/g), aqueous extract (42.02±0.36 mg/g) and n-Hexane (12.48±0.27 mg/g). The similar pattern in stem part was also observed, i.e. methanolic extracts (94.37±0.18 mg/g), ethyl acetate (44.31±0.45 mg/g), water (32.82±0.31 mg/g) and n-Hexane (8.33±0.21 mg/g). The antioxidant capacity of methanolic extract of both the part, i.e. leaf and stem were founded highest as IC50 values were 63.41±0.32, 81.69±0.40 respectively. The reducing power was also highest in the methanol extract of both parts. The result data conclude that the higher antioxidant as well as reducing power may be due to present phenolic contents.