BITS Faculty Publications
Permanent URI for this communityhttp://localhost:4000/handle/123456789/1867
Browse
14 results
Search Results
Item Resorption potential of endoflas powder with curcumin gel against endoflas for pulpectomy in primary mandibular molars: a one-year follow-up evidence-based case reports(Springer Nature, 2024-06) Mahesh, RadhakrishnanPediatric endodontics in the primary teeth is chemomechanical due to the difficulties encountered in cleaning and shaping the root canals having complex anatomical configurations. Not only are obturating materials having ideal properties to be used in the primary teeth, but also imparting an impervious hermetic seal to prevent the nidus of reinfection is highly pivotal for the success of pulpectomy. However, certain obturating materials contain one or the other components that are irritant to the periapical region and aggravates the inflammatory process. Hence, a paradigm shift has been witnessed with the evolution of herbal medicines having innumerable beneficial properties with a broad-spectrum action to replace those components causing irritation and inflammatory reactions in conventional obturating materials. One such herbal medicament is “curcumin” popularly known as the “golden herb,” which has a wide repertoire of medicinal properties due to its bioactive component and volatile oil “turmerone.” Endoflas is one of the suitable obturating agents used in the primary teeth for its high success rate, but the use of eugenol in it is associated with irritation of the periapical region and causes necrosis of bone and cementum. Hence, the present case report aims to assess the rate of resorption, resorption potential, and the periapical healing of a novel obturating material Endoflas powder with curcumin gel (EPCG) replacing the liquid eugenol against conventional Endoflas material for pulpectomy in primary mandibular molars.Item Determination of oxaliplatin and curcumin in combination via micellar HPLC and its method validation(OUP, 2022-03) Jain, AnkitA micellar-HPLC method was developed for the determination of oxaliplatin (OHP) and curcumin (CUR) employing a C18 column [4.6 × 250 mm, particle size (dp) = 5 µm] and diode array detector.Item Hyaluronic acid functionalized liposomes embedded in biodegradable beads for duo drugs delivery to oxaliplatin-resistant colon cancer(Elsevier, 2022-11) Jain, AnkitOxaliplatin (OHP) resistance is a major hurdle in the chemotherapeutic treatment of colorectal cancer (CRC). The present study aimed to formulate Eudragit S-100 (ES-100) coated alginate beads bearing drugs loaded targeted liposomes for simultaneous delivery of OHP and curcumin (CUR) to exert a synergistic therapeutic effect on OHP- resistant HT-29 cell lineItem UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: Method development, in-vitro and ex-vivo applications in topical delivery(Elsevier, 2020-01) Pandey, Murali Monohar; Singhvi, GautamCurcumin the extract obtained from the dried rhizome of turmeric, Curcuma longa is a hydrophobic phenol that delivers numerous pharmacological actions like anti-inflammatory, anti-microbial and anti-oxidant, anti-psoriasis, antidiabetic, anticancer. But curcumin has low bioavailability issues that accompany low aqueous solubility, further, when administered orally, >90% of the drug degrades rapidly in the alkaline medium. Administering the drug topically can bypass the problem as well as first-pass metabolism and therefore delivering the drug at the targeted site of action. Encapsulating curcumin in nanostructured lipid nanocarriers (NLC) is an excellent novel strategy. Further, these NLC provides both the controlled release and helps in the enhanced permeation of the drug through the skin's physiological barrier, stratum corneum. For the NLC characterization, a reliable method must be developed that can accurately and precisely determine the drug content in the formulation and also for its in-vitro and ex-vivo characterization. This experiment describes the analytical validation parameters described as per International Conference of Harmonization guidelines to develop a method using the UV–Visible spectroscopy. The method was developed in two solvent systems i.e. methanol and 6.4 pH phosphate buffer with 1.5% polysorbate 80. Methanol solvent was used for the determination of curcumin in the NLC formulation via determining the encapsulation efficiency and 6.4 pH phosphate buffer with 1.5% polysorbate 80 solvent was used for in-vitro and ex-vivo characterization of the developed NLC formulation (cream and gel). These methods were validated in response to linearity, the limit of detection, the limit of quantification, precision, accuracy, repeatability, and specificity.Item UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: Method development, in-vitro and ex-vivo applications in topical delivery(Elsevier, 2020-01) Pandey, Murali Monohar; Singhvi, GautamCurcumin the extract obtained from the dried rhizome of turmeric, Curcuma longa is a hydrophobic phenol that delivers numerous pharmacological actions like anti-inflammatory, anti-microbial and anti-oxidant, anti-psoriasis, antidiabetic, anticancer. But curcumin has low bioavailability issues that accompany low aqueous solubility, further, when administered orally, >90% of the drug degrades rapidly in the alkaline medium. Administering the drug topically can bypass the problem as well as first-pass metabolism and therefore delivering the drug at the targeted site of action. Encapsulating curcumin in nanostructured lipid nanocarriers (NLC) is an excellent novel strategy. Further, these NLC provides both the controlled release and helps in the enhanced permeation of the drug through the skin's physiological barrier, stratum corneum. For the NLC characterization, a reliable method must be developed that can accurately and precisely determine the drug content in the formulation and also for its in-vitro and ex-vivo characterization. This experiment describes the analytical validation parameters described as per International Conference of Harmonization guidelines to develop a method using the UV–Visible spectroscopy. The method was developed in two solvent systems i.e. methanol and 6.4 pH phosphate buffer with 1.5% polysorbate 80. Methanol solvent was used for the determination of curcumin in the NLC formulation via determining the encapsulation efficiency and 6.4 pH phosphate buffer with 1.5% polysorbate 80 solvent was used for in-vitro and ex-vivo characterization of the developed NLC formulation (cream and gel). These methods were validated in response to linearity, the limit of detection, the limit of quantification, precision, accuracy, repeatability, and specificity.Item Curcumin loaded nanostructured lipid carriers for enhanced skin retained topical delivery: optimization, scale-up, in-vitro characterization and assessment of ex-vivo skin deposition(Elsevier, 2020-09) Singhvi, Gautam; Roy, AniruddhaNanostructured lipid carriers (NLC) have become a promising drug delivery system for topical delivery of drugs. Delivery of lipophilic drugs with improved stability and entrapment efficiency is one of the foremost benefits of NLC based formulations. The objective of the present study was to improve the permeation of poorly soluble curcumin into topical skin layers for the treatment of chronic inflammatory disorder psoriasis and microbial mediated acne vulgaris. Hot emulsification followed by probe sonication method was employed for the preparation of the curcumin loaded NLC. Further, in-vitro and ex-vivo characterization was performed for designed NLC. The designed NLC showed a mean particle size 96.2 ± 0.9 nm, entrapment efficiency of 70.5 ± 1.65% and zeta potential of -15.2 ± 0.566 mV. Curcumin-NLC showed extended in-vitro release upto 48 hours, whereas free curcumin showed 100% drug release within 4 hours. Ex-vivo skin permeation studies revealed 3.24 fold improved permeation and skin retention in the case of curcumin loaded NLC gel compared to free curcumin gel. The cell viability studies demonstrated the formulation components showed no toxicity towards keratinocyte cells. In keratinocyte cells, improved cell uptake was observed for curcumin-NLC compared to free curcumin dispersion. The results suggested that the NLC based formulation had potential to improve the efficacy of curcumin.Item Curcumin loaded biomimetic composite graft for faster regeneration of skin in diabetic wounds(Elsevier, 2018-10) Mittal, AnupamaDiabetic wounds do not heal in an organized and timely manner due to poor blood glucose control and weakened immune system. In the present study, wound healing properties of curcumin were explored by designing and evaluating a biomimetic composite graft for skin regeneration in diabetic wounds. Curcumin was incorporated in gelatin solution by nanoprecipitation method in particle size of 311.3 ± 35.1 nm. Cytomodulin coupled porous PLGA microparticles (cPMS) were prepared, dispersed in curcumin loaded gelatin solution and cross-linked with glutaraldehyde (GTA) to form a composite graft (Gel-Cur-cPMS). Under SEM, cPMS showed porous structure and Gel-Cur(1%w/v)-cPMS(1%w/v) indicated interconnecting pores of gelatin hydrogel. Formulation stability at the application site was indicated by micro shrinkage temperature. Gel-Cur(1%w/v)-cPMS(1%w/v) absorbed 40% of additional fluid weight of its own weight and demonstrated a sustained release of the curcumin over a period of 7 days. Evaluation of wound healing properties of the composite graft in diabetic wound model indicated 75%, 68% and 63% of wound closure with Gel-Cur(1%w/v)-cPMS(1%w/v), Gel-Cur(1%w/v) and untreated control group respectively. Gel-Cur(1%w/v)-cPMS(1%w/v) demonstrated better and faster wound healing visually along with regenerating a skin of higher tensile strength than Gel-Cur(1%w/v) treated and untreated control group. These results demonstrate that the curcumin-loaded composite graft bears potential as a skin graft with wound healing properties in diabetic wounds.Item Curcumin as an Adjuvant to Breast Cancer Treatment(Bentham Science, 2015-06) Gaikwad, Anil BhanudasCancer is the second largest leading cause of death worldwide and breast cancer is the most prevailing cause of mortality among all cases of malignant neoplastic disease in adult females. The incidence rate of breast malignant neoplastic disease is predominantly higher in Western women, when compared to women in Asian nations. The definitive reason for this conflict is even unknown, but dietary factors have been conceived to account for approximately 30% of cancers in Western nations. It has been hypothesized that ethnicity, including use of a variety of spices in the food would be a major reason. Among all spices, turmeric (Curcuma longa) has been proven for its better anticancer potential. In this review different molecular mechanisms including cell cycle arrest; G0/G1 and/or G2/M phase cell cycle arrest by up-regulating Cdk inhibitor, p21/WAF/CIPI and p53, inhibition of transcriptional factors; NFΚB, AP-1, TNFα, IL, STAT-3, and PPAR-γ, downstream gene regulation; c-myc, Bcl-2, COX-2, NOS, Cyclin D1, TNFα, interleukins and MMP-9, growth factors; bFGF, EGF, GCSF, IL-8, PDGF, TGFα, TNF, VEGF and cell adhesion molecules; fibronectin, vitronectin, and collagen which are involved in angiogenesis and metastasis, alsothe effectiveness of curcumin, when given in combination with chemotherapeutics like cyclophosphamide, doxorubicin, mitomycin etc. in treating breast cancer have been reviewed.Item Biomimetic Oxidation of Curcumin with Hydrogen Peroxide Catalyzed by 5,10,15,20-Tetraarylporphyrinatoiron(III) Chlorides in Dichloromethane(J-Stage, 2003) Kumar, AnilThe biomimetic oxidation of curcumin, a main turmeric pigment with hydrogen peroxide catalyzed by different 5,10,15,20-tetraarylporphyrinatoiron(III) chlorides [TAPFe(III)Cl] in dichloromethane has been studied to give a C–C coupled curcumin dimer in 40—70% yield. The structure of the dimer has been elucidated by 1H-, 13C-NMR, IR and FAB-Mass spectroscopic data.Item Curcumin! Spicy panacea for all—Friend or foe(Elsiever, 2009-12-11) Marathe, SandhyaTurmeric, an “Indian gold” from Curcuma longa is a household spice in India. The essential component of the turmeric is curcumin and curcuminoids. This spice has managed to leave the household spice bottles and found its place in reputed herbal and medical outlets in beautifully packed bottles. Various pharmaceutical companies have invested in marketing curcumin as compressed tablets, capsules and powder. Various scientific research and clinical trials suggested its use in disorders ranging from Alzheimer's to cancer [1], [2]. Curcumin became panacea for all diseases. Experimental studies using various cell lines and animal models suggested the role of curcumin in possessing anti-inflammatory [3], anti-bacterial [4] and anti-cancer [5] activities. Curcumin have also been shown to lower the cholesterol by increasing the LDL-R [6]. One human clinical trial in Helicobacter pylori (H. pylori) infected patients suggested its role in bringing down the dyspepsia caused by H. pylori. However, curcumin did not clear or decrease the H. pylori burden [7]. The way curcumin signals and modulate the immune functions of various cell types gives an impetus to think that “Can curcumin be given blindly as therapy”? The reactive oxygen and nitrogen species (ROI and RNI) have been always targeted as “evil molecules”, forgetting that the same phagocyte oxidants, in fact are the first line of defense against plethora of intracellular pathogens.